4everyone Detection Kit B Lactobacillus acetotolerans.
The beer spoiling species L. acetotolerans is hard to cultivate and often can only be detected by real time PCR analysis. A contamination of beer with L. acetotolerans leads toextreme Acid production. Until today, only secondary contaminations in the filler area were identified as contamination sources.
Each kit contains all buffers for the analysis of liquid samples or colonies (DNA isolation), and additionally all reagents including primers and probes for real time PCR which are ready-made dried into the PCR tubes.
4everyone Detection Kits can be used in most real time PCR thermocyclers made for 0.1 mL PCR tubes. They are optimized for the Chai™ Open qPCR thermocycler which allows automated PCR analysis down to the final analysis of results, guided by the software which comes on board. There is no need to read-out any curves or lines manually. After the analysis has finished, the outcome is shown in a table format direct as positive or negative test result, even giving a quantification of positives in numbers (as Cq values).
If you are using a real time thermocycler with a different format, we have further kit product lines available (SO Detection Kits with 100 µL and TM Detection Kits with 200 µL PCR tubes, both for measurement from top through the cap) – please inquire. A compatibility list of different thermocyclers and recommended Detection Kit formats is shown here. As all kits include an internal inhibition control reaction besides the detection of the target microorganism, a 2 channel real time PCR thermocycler is needed (FAM and HEX/VIC).
4everyone Detection Kits are for research use in vitro only.
You can find data sheets and user manuals here.