Positive Result in Enrichment

Warning of Problems in Product Quality

Growth of bacteria, yeasts or molds in routine control are advance notice of product quality problems. But what is the damage potential? This is the essential question. Some bacteria or yeasts are proliferating in enrichment media while they are not able to grow in the beverage itself. So everything is only false alarm? Not at all – and nothing which you should ignore …

The Problem

In case of a product contamination, prompt assessment of the actual risk is necessary to avoid an invasion of other batches. The best hint to localize its source is given by identification of the product spoilers followed by their specific backtracing through the production line.

Solution Beer

In a brewery, positives are generally detected in enrichments. But from his experience the brewmaster knows that in enrichments some bacteria may grow up to detectable numbers more or less frequently, but there is no danger at any time for the product beer – the beer stays clean. Why?

In the closed container there are different conditions than in enrichment: There are barely no available nutrients in beer, there is no oxygen and usually storage is in a cool place. In contrast, beer spoilers find an optimized supply of nutrients in enrichment, and they are incubated at convenient temperature. The aim is to bring all potentially present microorganisms to proliferation within a short time to be able to reliably detect them. Therefore you can always expect – provided that the analysis covers a representative volume – a higher detection rate of contaminations in enrichments than in the finished beer. The results from routine control in a brewery are used to maintain the optimal hygiene status and to prevent growth of problem bacteria even within the production process. But nevertheless the real beer spoiling bacteria should always be differentiated, because only this type of result gives you knowledge about the actual product risk.

Samples from routine control are controlled for beer spoilers by use of FastOrange® B media – positives are clearly detectable after 3-7 days by color change from violet to yellow. By PCR analysis, beer spoilers are detected at an even earlier stage – 3 days after sampling. Besides, positives in all types of enrichments as lactobacilli and pediococci can be further differentiated.

To us, it is especially important that we detect the really beer spoiling Lactobacillus species only which are covered with our PCR analyses and Detection Kits. A false positive result would do more harm than good to the brewer.

An example from practice is Lactobacillus paracasei, a non- beer spoiling bacterium which is regarded as part of the accompanying flora in breweries. Although Lactobacillus paracasei usually does not grow even in lightly hopped beers, this species is proliferating fast in different enrichment media and shows a similar appearance as Lactobacillus casei. These two species are hardly distinguishable, even in microscopic examination. Here the PCR analysis is really helpful: With our PCR Kits and the PCR analysis, a simple and precise differentiation between L. casei and L. paracasei is available. We can clearly determine from a sample if there is harmless accompanying flora or beer spoiling bacteria.

In addition to the name of the product pests, our lab will provide you with a history of their occurrence and data on appropriate measures to kill them. We compare earlier findings from your plant with the current result and can thus create a history of the occurrence. This information provides you with a sound basis for risk assessment and specific traceability to the source of contamination. We also advise you on further steps to kill the pests and recommend prevention measures for future batches.

Solution Juice, Soft Drinks, Water

If bacteria, yeasts or molds occur in a product or during production, those must be differentiated as specifically as possible to allow causal research. As those are often microorganisms which are brought in with raw materials or from the production area, a broad spectrum of species may occur, and not all of those isolates can be tested by simple identification or screening analyses. The products often provide a rich nutrient supply so the growth limiting factors are only the lack of oxygen and – if applied – heat treatment.

Heat resistant bacteria as Alicyclobacillus or heat resistant molds are classified with our PCR Detection kits much easier and faster than with the traditional enrichment methods. The best way to identify isolates from the diversity of microorganisms which may occur in the periphery of a production line is the use of molecular biology analysis as DNA sequencing.

As a result of identification, our lab delivers besides the name of the microorganism also a history about its usual habitat together with data about appropriate destroying measures. This information is your fundamental base for risk assessment and for specific backtracing actions through the production line. We also give advice about further steps to eliminate the spoilers and about suitable prevention measures for further batches.

PIKA Services

Microbiology Analyses

We are fast and competent in doing routine analyses as enrichments, determination of colony counts and the differentiation of microorganisms – service and quality are most important for us. Of course we respond to your requirements and always deliver prompt and reliable results – if necessary, even through weekends.

Get more information …

DNA Analyses with PCR

Spoilt product or positive enrichments are further discriminated – fast and specific by PCR analyses the same day as the samples are received. We further identify unknown bacteria, yeasts or molds from product or the production area within 3 to 5 days by DNA sequencing.

Get more information …

Broad Process Consultancy

Our work does not end at the analysis result, we further advise you how to deal with a contamination and propose the next steps to minimize the risk for further batches in your production line. You can also count on us when optimizing your routine quality control measures.

Get more information …

PIKA Products

FastOrange B

PIKA FastOrange is a nutrient medium which was developed especially for the detection of beer spoiling microorganisms in beer and through the whole production process. Following the one-for-all principle, it can be used for enrichment of all brewery samples.

Get more information …

FastOrange Yeast

The enrichment medium FastOrange Yeast was specifically developed for use in the food and beverage industry as well as in breweries and wineries. In this medium both yeasts and molds are growing.

Get more information …

PCR Detection Kits

We are offering PCR Kits for detection and identification of bacteria, yeasts and molds. Each kit is a complete package ready-to-use: It contains material for DNA isolation and a PCR module including PCR tubes thus covering the whole analysis.

Get more information …