Microbial Growth Surviving Heat Treatment

Routine Control must be Adjusted to include Heat Resistant Microorganisms

By heat treatment – flash or tunnel pasteurisation – microorganisms in the product are destroyed and the shelf-life of the product is extended. Commonly used procedures are optimized for the typically occurring microorganisms. In breweries, the settings for time and temperature are aiming on the inactivation of yeasts and bacteria. Nevertheless live microorganisms reach the product – why?

The Problem

The effect of thermal processes is stated as reduction of the microbial count in decimal values. During heat treatment at a defined temperature over a determined time, typically a certain percentage of present microorganisms is killed. The conditions of heat treatment as well as the killing rates are depending on the composition of the treated product, but another crucial factor is the species of the microorganism to be destroyed. The killing rate should ideally be 100%, but usually this rate is not achieved. On one hand the heat distribution often is not equal and so a part of the product may not be exposed to the theoretical dosage. On the other hand some microorganisms may resist considerably high temperatures – higher than the treated product itself does. For these reasons, routine control has always to cover heat resistant microorganisms, especially for those products which are containing fruit and sugar.

The Solutions

If microorganisms survive the heat treatment, then either the pasteuriser had an error, or its settings were wrong – or there were heat resistant microorganisms present which are common on fruit and plants. These are including typical spore producing bacteria as Bacillus species or Clostridia, but also heat resistant molds as some Penicillius species, Byssochlamys, Neosartorya, or Talaromyces. Through heat treatment usually the vegetative forms are killed, but the spores survive. And those are awakened by the impact of heat, they germinate and further proliferate, producing spoilage in the finished product.

The detection of those microorganisms in raw materials and before heat treatment is difficult if vegetative cell material is not present in a detectable amount – the spores themselves are hardly detectable. In juice and soft drink production analysis, such procedures which start with a heat treatment of the sample before the actual analysis by enrichment begins are state-of-the-art: The spore germination is induced and after that their detection is possible.

Breweries which more and more are producing mixed drinks have to follow, for them today there are no established routine methods available. We combine our experiences from microbiology in fruit drinks with our knowledge from the brewery analytics and can support you both with quick analyses as well as in the planning and establishing of analysis procedures for your products in your own lab.

PIKA Services

Microbiology Analyses

We are fast and competent in doing routine analyses as enrichments, determination of colony counts and the differentiation of microorganisms – service and quality are most important for us. Of course we respond to your requirements and always deliver prompt and reliable results – if necessary, even through weekends.

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DNA Analyses with PCR

Spoilt product or positive enrichments are further discriminated – fast and specific by PCR analyses the same day as the samples are received. We further identify unknown bacteria, yeasts or molds from product or the production area within 3 to 5 days by DNA sequencing.

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Broad Process Consultancy

Our work does not end at the analysis result, we further advise you how to deal with a contamination and propose the next steps to minimize the risk for further batches in your production line. You can also count on us when optimizing your routine quality control measures.

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PIKA Products

FastOrange B

PIKA FastOrange is a nutrient medium which was developed especially for the detection of beer spoiling microorganisms in beer and through the whole production process. Following the one-for-all principle, it can be used for enrichment of all brewery samples.

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FastOrange Yeast

The enrichment medium FastOrange Yeast was specifically developed for use in the food and beverage industry as well as in breweries and wineries. In this medium both yeasts and molds are growing.

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PCR Detection Kits

We are offering PCR Kits for detection and identification of bacteria, yeasts and molds. Each kit is a complete package ready-to-use: It contains material for DNA isolation and a PCR module including PCR tubes thus covering the whole analysis.

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