4e For everyone Detection Kits

Microbiology as easy as a child’s play

Get the fast and easy “go/no-go” test and monitor beer and wine spoilers throughout your whole production process. Microbiology testing with 4e® For everyone Detection Kits and Open qPCR user friendly real time PCR at a low budget. Detect all real beer spoilers as lactic acid bacteria including Lactobacillus acetotolerans, and super-attenuating yeasts Saccharomyces cerevisiae var. diastaticus or Brettanomyces. No need to check if lines are invisible, the quantitative results from 4e® Detection Kits show up automatically when the run has finished.

The PIKA Approach

4e® For everyone Detection Kits are made for everybody: You don’t need to be a specialist to do microbiology testing with 4e real time PCR. Each analysis which you can do by yourself saves you time and money – all our tests are low cost, simple and effective for use even in small breweries. PIKA Weihenstephan is working in brewery PCR since the year 2000, and we are constantly refining our product range close to the brewing industry. Our goal is making the best microbiology methods available to breweries of all sizes.

Our Policy

All PIKA Weihenstephan products derive from developments in our own service lab – if we don’t like it, we don’t sell it. Our policy is simple: Always to produce and deliver stable and reliable products, with fairness to the customer and responsibility for the environment, all at an affordable price: 4e® For everyone Detection Kits ship at ambient temperature, packaged in paper boxes, without styrofoam. No freeze and thaw cycles are needed, simply store the kits at your lab in the fridge up to one year.

4e For everyone Detection Kits – Detection and Identification of Microorganisms

With 4e® For everyone Detection Kits, we deliver state of the art microbiology testing to breweries of all sizes. Deriving from real time PCR, 4e® Detection Kits are today the easiest low budget analysis system for objective and quantitative beer spoiler detection.

The Advantages:

  • Simple and fast: detect all real beer spoilers in one test
  • Easy to use: no specific skills needed
  • Universal: one process for all samples
  • Objective: quantitative results in numbers, independent from user
  • Practical: run different tests together side by side
  • Small: Minimal lab space needed

Fields of application:

  • Routine QC in breweries and wineries
  • Simple go/no-go check even for small companies
  • Process monitoring
  • Backtracing of contaminations
  • Identification and verification of contaminants
  • Differentiation of spoiler species, e.g. Lactobacillus acetotolerans or Saccharomyces cerevisiae var. diastaticus

Specific and Precise

All product relevant microorganisms are detected within one single test. The detection limit is some 100 microorganisms which cannot be reached with any other method in the same time. Bacteria or yeasts from a sample are classified according to their spoilage potential and identified.

Safe and robust

Our PCR kits are containing all components which are necessary for the analysis pre-dosed and dried in the PCR tubes. So it is guaranteed that all components are always included in the correct ratio in each test. We ship at ambient temperature, PIKA’s PCR kits are stable even at room temperature.

Fast and Cost Efficient

Processing of samples takes only 15 minutes. The results from thermocycling are available within 75 minutes. All our PCR kits are matching to each other. This allows that different analyses as detection of bacteria and yeasts may be done at the same time, efficient and cost saving.

Further PIKA Products and Services

Competence in microbiology with a broad background: The innovative products from PIKA are derived from applications of our own in-house analytics and services for producers. All our products from the whole portfolio are perfectly matched. PIKA products are outstanding in easy handling and extreme reliability, our services in short processing time and top quality at affordable prices.

FastOrange Enrichment Media

Developed by us to protect beer and many other beverages from microbial spoilage: Every brewery and beverage producer can use PIKA FastOrange for routine control throughout the whole process line. FastOrange is available in different varieties, according to the capabilities and equipment of your own business.

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DNA Isolation Kits

Developed for the direct use in a production laboratory: Optimized for the extraction of genomic DNA from beer, wine and other beverages as well as from malt, grain and cereals – for the detection of bacteria, yeasts and molds. Cost saving thanks to simple and fast handling and a minimum of equipment needs.

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DNA-Analyses with PCR

Spoilt product or positive enrichments are further discriminated – fast and specific by PCR analyses the same day as the samples are received. We further identify contaminations by bacteria, yeast or molds in a product or from the production periphery within 3 to 5 days by DNA sequencing.

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Broad Process Consultancy

Our work does not end with the analysis result, we further advise you how to deal with a contamination and which are the next steps you should make to minimize the risk for further batches in your production line. You can also count on us when optimizing your routine quality control measures.

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